Journal: Experimental and Therapeutic Medicine

Article Title: Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions

doi: 10.3892/etm.2023.12360

Figure Lengend Snippet: Effect of a 3-day decrease in PTBP1 protein level in HT22 cells on cell differentiation and maturation. (A) Representative western blotting of MAP2, PTBP1, βIII-Tubulin, NeuN and GAPDH (n=4 per group). Quantitative statistical analysis results of (B) MAP2, (C) PTBP1, (D) βIII-Tubulin, (E) NeuN. (F) Representative immunofluorescence staining images and quantitative statistical analysis results of (G) βIII-Tubulin and (H) PTBP1. (I) Representative immunofluorescence staining images and quantitative statistical analysis results of (J) NeuN and (K) PTBP1. (L) Representative immunofluorescence staining images and quantitative statistical analysis results of (M) MAP2 and (N) PTBP1 (n=3 per group). Scar bar, 50 µm. ** P<0.01, *** P<0.001 and **** P<0.0001. PTBP1, polypyrimidine tract-binding protein 1; MAP2, microtubule-associated protein 2; NC, negative control; si, small interfering; ns, not significant.

Article Snippet: The cover slides were blocked with antibody dilution agent (cat. no. A1800; Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 30 min and then incubated overnight at 4˚C with primary antibodies: Rabbit anti-PTBP1 antibody (1:500; cat. no. 101043-T46; Sino Biological, Inc.), mouse anti-PTBP1 antibody (1:50; cat. no. sc-515282; Santa Cruz Biotechnology, Inc.), mouse anti-βIII-Tubulin antibody (1:200; cat. no. Sc-80016; Santa Cruz Biotechnology, Inc.) and rabbit anti-NeuN antibody (1:200; cat. no. AF1072; Beyotime Institute of Biotechnology).

Techniques: Cell Differentiation, Western Blot, Immunofluorescence, Staining, Binding Assay, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions

doi: 10.3892/etm.2023.12360

Figure Lengend Snippet: Effect of a 5 day decrease in PTBP1 protein level in HT22 cells on cell differentiation and maturation. (A) Representative western blotting of MAP2, PTBP1, βIII-Tubulin, NeuN and GAPDH (n=4 per group). Quantitative statistical analysis results of (B) MAP2, (C) PTBP1, (D) βIII-Tubulin and (E) NeuN. (F) Representative immunofluorescence staining images and quantitative statistical analysis results of (G) βIII-Tubulin and (H) PTBP1. (I) Representative immunofluorescence staining images and quantitative statistical analysis results of (J) NeuN and (K) PTBP1. (L) Representative immunofluorescence staining images and quantitative statistical analysis results of (M) MAP2 and (N) PTBP1 (n=3 per group). Scar bar, 50 µm. ** P<0.01, *** P<0.001 and **** P<0.0001. PTBP1, polypyrimidine tract-binding protein 1; MAP2, microtubule-associated protein 2; NC, negative control; si, small interfering; ns, not significant.

Article Snippet: The cover slides were blocked with antibody dilution agent (cat. no. A1800; Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 30 min and then incubated overnight at 4˚C with primary antibodies: Rabbit anti-PTBP1 antibody (1:500; cat. no. 101043-T46; Sino Biological, Inc.), mouse anti-PTBP1 antibody (1:50; cat. no. sc-515282; Santa Cruz Biotechnology, Inc.), mouse anti-βIII-Tubulin antibody (1:200; cat. no. Sc-80016; Santa Cruz Biotechnology, Inc.) and rabbit anti-NeuN antibody (1:200; cat. no. AF1072; Beyotime Institute of Biotechnology).

Techniques: Cell Differentiation, Western Blot, Immunofluorescence, Staining, Binding Assay, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions

doi: 10.3892/etm.2023.12360

Figure Lengend Snippet: Effect of a 3 day decrease in PTBP1 protein level in MA cells on cell differentiation. (A) Representative western blotting of MAP2, PTBP1, βIII-Tubulin, NeuN and GAPDH (n=4 per group). Quantitative statistical analysis results of (B) MAP2, (C) PTBP1, (D) βIII-Tubulin and (E) NeuN. (F) Representative immunofluorescence staining images and quantitative statistical analysis results of (G) βIII-Tubulin and (H) PTBP1. (I) Representative immunofluorescence staining images and quantitative statistical analysis results of (J) NeuN and (K) PTBP1. (L) Representative immunofluorescence staining images and quantitative statistical analysis results of (M) MAP2 and (N) PTBP1 (n=3 per group). Scar bar, 50 µm. *** P<0.001 and **** P<0.0001. PTBP1, polypyrimidine tract-binding protein 1; MAP2, microtubule-associated protein 2; NC, negative control; si, small interfering; ns, not significant; MA, mouse astrocyte.

Article Snippet: The cover slides were blocked with antibody dilution agent (cat. no. A1800; Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 30 min and then incubated overnight at 4˚C with primary antibodies: Rabbit anti-PTBP1 antibody (1:500; cat. no. 101043-T46; Sino Biological, Inc.), mouse anti-PTBP1 antibody (1:50; cat. no. sc-515282; Santa Cruz Biotechnology, Inc.), mouse anti-βIII-Tubulin antibody (1:200; cat. no. Sc-80016; Santa Cruz Biotechnology, Inc.) and rabbit anti-NeuN antibody (1:200; cat. no. AF1072; Beyotime Institute of Biotechnology).

Techniques: Cell Differentiation, Western Blot, Immunofluorescence, Staining, Binding Assay, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions

doi: 10.3892/etm.2023.12360

Figure Lengend Snippet: Effect of a 5 day decrease in PTBP1 protein level in MA cells on cell differentiation. (A) Representative western blotting of MAP2, PTBP1, βIII-Tubulin, NeuN and GAPDH (n=4 per group). Quantitative statistical analysis results of (B) MAP2, (C) PTBP1, (D) βIII-Tubulin and (E) NeuN. (F) Representative immunofluorescence staining images and quantitative statistical analysis results of (G) βIII-Tubulin and (H) PTBP1. (I) Representative immunofluorescence staining images and quantitative statistical analysis results of (J) NeuN and (K) PTBP1. (L) Representative immunofluorescence staining images and quantitative statistical analysis results of (M) MAP2 and (N) PTBP1 (n=3 per group). Scar bar, 50 µm. *** P<0.001 and **** P<0.0001. PTBP1, polypyrimidine tract-binding protein 1; MAP2, microtubule-associated protein 2; NC, negative control; si, small interfering; ns, not significant; MA, mouse astrocyte.

Article Snippet: The cover slides were blocked with antibody dilution agent (cat. no. A1800; Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 30 min and then incubated overnight at 4˚C with primary antibodies: Rabbit anti-PTBP1 antibody (1:500; cat. no. 101043-T46; Sino Biological, Inc.), mouse anti-PTBP1 antibody (1:50; cat. no. sc-515282; Santa Cruz Biotechnology, Inc.), mouse anti-βIII-Tubulin antibody (1:200; cat. no. Sc-80016; Santa Cruz Biotechnology, Inc.) and rabbit anti-NeuN antibody (1:200; cat. no. AF1072; Beyotime Institute of Biotechnology).

Techniques: Cell Differentiation, Western Blot, Immunofluorescence, Staining, Binding Assay, Negative Control